
84
H Anhang
I Abbildungen
Abb. H1: Alignment; Aminosäuresequenzen von RhoA, RhoB, RhoC, Rac1, RhoG, Cdc42, TC10, RhoD und
RalA
Mutanten: konstit.akt. / dom.neg. Switch I Region
RhoA-Nummerierung 1 14 19 54
RhoA -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVE
RhoB -------------------MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVE
RhoC -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVE
Rac1 ---------------------MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVN
RhoG ---------------------MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVN
Cdc42---------------------MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYT
TC10 -------MPGAGRSSMAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYL
RhoD -------MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVH
RalA ----------MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQ
Switch II Region
55 63 126
RhoA LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRNDEH
RhoB LALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCP-NVPIILVANKKDLRSDEH
RhoC LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRQDEH
Rac1 LGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCP-NTPIILVGTKLDLRDDKD
RhoG LNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCP-DVPILLVGTKKDLRAQPD
Cdc42LGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCP-KTPFLLVGTQIDLRDDPS
TC10 LGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAP-NVPFLLIGTQIDLRDDPK
RhoD LQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCK-GVPIIVVGCKIDLRKDKV
RalA IDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQ
Insert Reg. Isoprenylierung
127 191
RhoA TRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT-KDGVREVFEMATRAALQ-ARRGKKK-------SGCL
RhoB VRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKT-KEGVREVFETATRAALQ-KRYGSQNGCI----NCCK
RhoC TRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKT-KEGVREVFEMATRAGLQ-VRKNKRR-------RGCP
Rac1 TIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALT-QRGLKTVFDEAIRAVLC-PPPVKKRKRK------CL
RhoG TLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSALQ-QDGVKEVFAEAVRAVLN-PTPIK-RGRS------CI
Cdc42TIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALT-QRGLKNVFDEAILAALE-PPE-TQPKRK------CC
TC10 TLARLNDMKEKPICVEQGQKLAKEIGACCYVECSALT-QKGLKTVFDEAIIAILT-PKKHTVKKRIGSRCINCC
RhoD LVNNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARL-HDNVEAVFQEAAEVALSSRRHNFWRRIT----QNCC
RalA VSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMR-EIRARKMEDSKEKNGKKKRKSLAKRIR-----ERCC
192
RhoA VL------------------------
RhoB VL------------------------
RhoC IL------------------------
Rac1 LL------------------------
RhoG LL------------------------
Cdc42IF------------------------
Tc10 LIT-----------------------
RhoD LAT-----------------------
RalA IL------------------------
Mutanten: konstit.akt. / dom.neg. Switch I Region
RhoA-Nummerierung 1 14 19 54
RhoA -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVE
RhoB -------------------MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVE
RhoC -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVE
Rac1 ---------------------MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVN
RhoG ---------------------MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVN
Cdc42---------------------MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYT
TC10 -------MPGAGRSSMAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYL
RhoD -------MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVH
RalA ----------MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQ
Mutanten: konstit.akt. / dom.neg. Switch I Region
RhoA-Nummerierung 1 14 19 54
RhoA -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVE
RhoB -------------------MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVE
RhoC -------------------MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVE
Rac1 ---------------------MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVN
RhoG ---------------------MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVN
Cdc42---------------------MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYT
TC10 -------MPGAGRSSMAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYL
RhoD -------MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVH
RalA ----------MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQ
Switch II Region
55 63 126
RhoA LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRNDEH
RhoB LALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCP-NVPIILVANKKDLRSDEH
RhoC LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRQDEH
Rac1 LGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCP-NTPIILVGTKLDLRDDKD
RhoG LNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCP-DVPILLVGTKKDLRAQPD
Cdc42LGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCP-KTPFLLVGTQIDLRDDPS
TC10 LGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAP-NVPFLLIGTQIDLRDDPK
RhoD LQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCK-GVPIIVVGCKIDLRKDKV
RalA IDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQ
Switch II Region
55 63 126
RhoA LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRNDEH
RhoB LALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCP-NVPIILVANKKDLRSDEH
RhoC LALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCP-NVPIILVGNKKDLRQDEH
Rac1 LGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCP-NTPIILVGTKLDLRDDKD
RhoG LNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCP-DVPILLVGTKKDLRAQPD
Cdc42LGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCP-KTPFLLVGTQIDLRDDPS
TC10 LGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAP-NVPFLLIGTQIDLRDDPK
RhoD LQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCK-GVPIIVVGCKIDLRKDKV
RalA IDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQ
Insert Reg. Isoprenylierung
127 191
RhoA TRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT-KDGVREVFEMATRAALQ-ARRGKKK-------SGCL
RhoB VRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKT-KEGVREVFETATRAALQ-KRYGSQNGCI----NCCK
RhoC TRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKT-KEGVREVFEMATRAGLQ-VRKNKRR-------RGCP
Rac1 TIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALT-QRGLKTVFDEAIRAVLC-PPPVKKRKRK------CL
RhoG TLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSALQ-QDGVKEVFAEAVRAVLN-PTPIK-RGRS------CI
Cdc42TIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALT-QRGLKNVFDEAILAALE-PPE-TQPKRK------CC
TC10 TLARLNDMKEKPICVEQGQKLAKEIGACCYVECSALT-QKGLKTVFDEAIIAILT-PKKHTVKKRIGSRCINCC
RhoD LVNNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARL-HDNVEAVFQEAAEVALSSRRHNFWRRIT----QNCC
RalA VSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMR-EIRARKMEDSKEKNGKKKRKSLAKRIR-----ERCC
Insert Reg. Isoprenylierung
127 191
RhoA TRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT-KDGVREVFEMATRAALQ-ARRGKKK-------SGCL
RhoB VRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKT-KEGVREVFETATRAALQ-KRYGSQNGCI----NCCK
RhoC TRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKT-KEGVREVFEMATRAGLQ-VRKNKRR-------RGCP
Rac1 TIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALT-QRGLKTVFDEAIRAVLC-PPPVKKRKRK------CL
RhoG TLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSALQ-QDGVKEVFAEAVRAVLN-PTPIK-RGRS------CI
Cdc42TIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALT-QRGLKNVFDEAILAALE-PPE-TQPKRK------CC
TC10 TLARLNDMKEKPICVEQGQKLAKEIGACCYVECSALT-QKGLKTVFDEAIIAILT-PKKHTVKKRIGSRCINCC
RhoD LVNNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARL-HDNVEAVFQEAAEVALSSRRHNFWRRIT----QNCC
RalA VSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMR-EIRARKMEDSKEKNGKKKRKSLAKRIR-----ERCC
192
RhoA VL------------------------
RhoB VL------------------------
RhoC IL------------------------
Rac1 LL------------------------
RhoG LL------------------------
Cdc42IF------------------------
Tc10 LIT-----------------------
RhoD LAT-----------------------
RalA IL------------------------
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